Molecular Weight Markers for Gel Electrophoresis

NOTE: Two types of molecular weight (MW) standards are routinely used. The Lambda/HindIII and
PhiX174/HaeIII MW standards provide a useful reference for calculating molecular weights of large
and small DNA fragments, respectively, after electrophoretic separation; the ìinternal MW standardsî
provide a means for normalizing fragment migration distances within each lane to facilitate comparisons
between lanes on the same or different luminographs in fingerprinting studies.
END-LABELED LAMBDA (l) DNA AS A MOLECULAR WEIGHT
STANDARD FOR LUMINOGRAPHS:
DIGESTION OF ...

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Lyophilization

1. Harvest leaves from greenhouse or field grown plants. It is preferable to use young leaves without necrotic
areas or lesions, although older leaves which are not senescent may be used.
2. If the midrib is thick and tough, remove it. Cut or fold leaves into 10-15 cm sections and place in a fiberglass
screen mesh bag along with the tag identifying the sample (Aluminum foil or paper bags may be substituted if
holes are punched to ...

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Isolation of Plasmid Inserts

1. Prepare bulk digestion mix using the appropriate enzyme (PstI, SalI, etc.) and correct enzyme buffer.
STOCK                                                                        [FINAL]                                                 ...

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