Molecular Weight Markers for Gel Electrophoresis

NOTE: Two types of molecular weight (MW) standards are routinely used. The Lambda/HindIII and
PhiX174/HaeIII MW standards provide a useful reference for calculating molecular weights of large
and small DNA fragments, respectively, after electrophoretic separation; the ìinternal MW standardsî
provide a means for normalizing fragment migration distances within each lane to facilitate comparisons
between lanes on the same or different luminographs in fingerprinting studies.
END-LABELED LAMBDA (l) DNA AS A MOLECULAR WEIGHT
STANDARD FOR LUMINOGRAPHS:
DIGESTION OF ...

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Isolation of Plasmid Inserts

1. Prepare bulk digestion mix using the appropriate enzyme (PstI, SalI, etc.) and correct enzyme buffer.
STOCK                                                                        [FINAL]                                                 ...

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