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(based on protocols from Vos et al. 1995. Nuc. Acid Res. 23:4407-4414, Greg Penner, AAFC, Winnipeg and the
Digoxigenin system of Enrico Perotti, CIMMYT)

NOTES:
This AFLP protocol has been optimized for hexaploid wheat but has worked very well for maize, rye, tetraploid
durum wheat and Tripsacum. The use of PstI instead of EcoRI is especially useful for hexaploid wheat due to
the very large genome size and the very high level of repetitive sequences. ...

Coming Soon…

Both Balantidium coli trophozoites and cysts are found in stool. Trophozoites are characterized by: their large size (40 µm to 200 µm), the presence of cilia on the cell surface, a cytostome, and a bean shaped macronucleus which is often visible and a smaller, less conspicuous micronucleus. Cysts are seen less frequently and range in size from 50 µm to 70 µm. B. coli can also invade tissue.

NOTE: Two types of molecular weight (MW) standards are routinely used. The Lambda/HindIII and
PhiX174/HaeIII MW standards provide a useful reference for calculating molecular weights of large
and small DNA fragments, respectively, after electrophoretic separation; the ìinternal MW standardsî
provide a means for normalizing fragment migration distances within each lane to facilitate comparisons
between lanes on the same or different luminographs in fingerprinting studies.
END-LABELED LAMBDA (l) DNA AS A MOLECULAR WEIGHT
STANDARD FOR LUMINOGRAPHS:
DIGESTION OF ...