(based on protocols from Vos et al. 1995. Nuc. Acid Res. 23:4407-4414, Greg Penner, AAFC, Winnipeg and the
Digoxigenin system of Enrico Perotti, CIMMYT)
This AFLP protocol has been optimized for hexaploid wheat but has worked very well for maize, rye, tetraploid
durum wheat and Tripsacum. The use of PstI instead of EcoRI is especially useful for hexaploid wheat due to
the very large genome size and the very high level of repetitive sequences. ...