Search Results for "Met"


  • DNA Quantification Using the TKO 100 MiniFluorometer
    1. Turn on the fluorometer and allow it to warm up for at least 15 minutes. 2. Prepare the amount needed of working dye solution and cover with foil. CALIBRATION 3. Pipette 2 ml of working dye into the glass cuvette. Wipe the cuvette and insert into the well (always place the cuvette in the same ori...
  • 2D electrophoresis principles and methods
    Preface “Proteomics” is the large-scale screening of the proteins of a cell, organism or biological fluid, a process which requires stringently controlled steps of sample preparation, 2-D electrophoresis, image detection and analysis, spot identification, and database searches. The core technol...
  • Incorporation of Digoxigenin-dUTP into Plasmid Inserts Using PCR
    1. Prepare a bulk reaction mix containing all the components listed below except plasmid. [FINAL]                                                     2.5% Dig                                                             5.0% Dig STOCK       ...
  • Incorporation of Digoxigenin-dUTP into DNA Using Nick Translation
    1. Prepare a bulk mix of all chemicals except DNA: STOCK                                                          [FINAL]                                                               (2.5% Dig)50 μl RXN 10 X NT  Buffer             ...
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