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• Molecular Weight Markers for Gel Electrophoresis
  NOTE: Two types of molecular weight (MW) standards are routinely used. The Lambda/HindIII and PhiX174/HaeIII MW standards provide a useful reference for calculating molecular weights of large and small DNA fragments, respectively, after electrophoretic separation; the ìinternal MW standardsî provi...

• Lyophilization
  1. Harvest leaves from greenhouse or field grown plants. It is preferable to use young leaves without necrotic areas or lesions, although older leaves which are not senescent may be used. 2. If the midrib is thick and tough, remove it. Cut or fold leaves into 10-15 cm sections and place in a fibergl...

• GEL ELECTROPHORESIS OF RNA AND NORTHERN BLOTTING
  REAGENTS: 10xGel running buffer: 0.4M MOPS, pH7.0/ 0.1M NaOAc/ 10mM EDTA Morpholinopropanesulfonic acid                                                   41.9g Na acetate.3H2O                                                                   ...

• 2D electrophoresis principles and methods
  Preface “Proteomics” is the large-scale screening of the proteins of a cell, organism or biological fluid, a process which requires stringently controlled steps of sample preparation, 2-D electrophoresis, image detection and analysis, spot identification, and database searches. The core technol...